stratmannlab :: protocols

Animal Anestheisa
Rat Anesthesia (Word .doc)

Apoptosis
Modified from Vesna Jevtovic-Todrovic-test (Word .doc)
Modified from Vesna Jevtovic-Todrovic (Word .doc)
Tunnel on slides (Word .doc)

Brain Slicing
Cutting Brains - Jialing (Word.doc)
Gelatin-Embed Protocol (Word .doc)
Sucrose sink (Word .doc)
Sucrose sink (Word .doc)

BrDU Labeling
BrDU Labeling (Word .doc)

Electrophysiology

Hippcampal slice culture
Notes from Jonathan (Word .doc)
Slice Culture Protocol (Word .doc)

Imaging
Confocal Stereology (Word .doc)

 

Immunocytochemistry
Double Staining Arturo Style - Day 1 (Word .doc)
Double Staining Arturo Style - Day 2 (Word .doc)
Double Staining Arturo Style - Day 3 (Word .doc)
Double Staining Jialing Style - Day 1 (Word .doc)
Double Staining Jialing Style - Day 2 (Word .doc)
Double Staining Jialing Style - Day 3 (Word .doc)
Double Staining - non BrDU (Word .doc)
Greg Double Staining BrDU (Word .doc)
Shetty non-BrDU double stains (Word .doc)
Sox-2 Ki-67 Shetty (Word .doc)
Staining Proliferation Experiments Day 1 (Word .doc)
Staining Proliferation Experiments Day 2 (Word .doc)

Neurobehavioral Tests
Cumulative Distance, etc (Word .doc)
Probe Trials Sheet Static (Excel .xls)
Rehan's WM setup protocol (Word .doc)
Rehan's WM Excel Design Template (Word .doc)
Water Maze neonates + 8 mths 4 h iso (Excel .xls)
Water Maze Protocol (Word .doc)

   
   

 

Perfusion
Perfusion of Rats (Word .doc)

Solutions
PBS Recipe (Word .doc)
Various Solutions (Word .doc)

 

Stem Cell Culture
Passaging of Neuro Stem Cells (.doc)
Published Protocol from Jeff (.pdf)

 
University of California, San Francisco
Department of Anesthesia, Room U271
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